gmuB [2018-06-22 18:09:57]

glucomannan-specific phosphotransferase system, EIIB component of the PTS

locus: BSU05810

pI: 4.72

mw: 11.30 kDa

protein length: 103 aa

gene length: 309 bp

function: glucomannan uptake and phosphorylation

product: glucomannan-specific phosphotransferase system EIIB component

essential: no

synonyms: ydhM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

Gene

Coordinates: 626,622 → 626,933

The protein

Paralogous protein(s)

• [protein|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|LicB]

Structure

• [PDB|4MGE] (from B. anthracis, 46% identity)

Expression and Regulation

Operons

• genes: [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
  description: [Pubmed|18177310]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]

Biological materials

Mutant

• MGNA-C188 (ydhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2186 NBRP B. subtilis, Japan]
• BKE05810 (Δ[gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA
• BKK05810 (Δ[gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA

References

• 18177310,10627040,22900538,20817675